Home Registration BABT BABGE   Web-link Gallery Contact
 
 

DEPARTMENT OF BIOTECHNOLOGY

Bangladesh Agricultural University, Mymensingh

Syllabus for B. Sc. Ag. (Hons.); Level-1; Semester-II Course No: BTech 121 Course Title: BASIC

BIOTECHNOLOGY-Theory (Elective)

Credit hrs: 2, Contact hours: 2

  1. Introduction: Concept, importance, scope, limitations and branches of biotechnology.

  2. Basic requirements: Media composition, aseptic manipulation and in vitro microenvironment.

  3. Cells: Composition, cellular classification, cell cycle

    1. Cell and Tissue Culture Techniques:

      1. Nodal culture, meristem culture and embryo culture.

      2. Protoplast isolation, culture, and somatic hybridization.

      3. Haploid production through anther, pollen, ovary and ovule culture.

-In vitro somaclonal and gametoclonal variations, ployploidization.

-
Plant regeneration from callus and cell suspension cultures through somatic embryogenesis.

-
Animal and fish cell technology, embryo transfer technology (ETT), multiple ovulation embryo transfer (MOET).

-In vitro production of secondary metabolites.

  1. DNA Structure: Modern concept of gene, Watson and Crick model of DNA.

  2. Genetic Engineering: Vectors, restriction endonuclease, ligase, cDNA. Methods of genetic engineering. Promoter, marker and reporter genes.

Text Books/References

Alberts, B., D. Bray, J. Lewis, M. Raff, K. Roberts and J. D. Watson. 1989. Molecular Biology of the

Cell. 2nd Edition. Garland Pub. Inc., New York. Alcamo, I. E. 2004. Schaum’s Outlines: Microbilogy. The McGraw-Hill Companies, Inc., New York. Brown, T. A. 1998. Recombinant DNA. Academic Press, London. Dixon, R. A. 1987. Plant Cell Culture. IRL Press, Washington D.C. Elrod, S. and W. Stanasfield. 2003. Schaum’s Outlines: Genetics. 4th Edition. Tata McGraw-Hill

Publishing Comp., Germany. Freifelder, D. 2004. Molecular Bilogy. 2nd Edition. Jones and Bartlett Pub., Inc., USA. Gamborg, O. L. and G. S. Phillips. 1995. Plant Cell Tissue and Organ Culture. Fundamental Methods,

Narosa Pub. House, New Delhi. Lewin, B. 2004. Genes VIII. Pearson Prentice Hall Pub., NJ 07458. Naryanaswarmy, S. 1994. Plant Cell and Tissue Culture, Tata McGraw-Hill Pub., New Delhi. Primrose, S. B., R. M. Twyman and R. W. Old. 2003. Principles of Gene Manipulation. 6th Edition.

Blackwell Pub. Com., Germany. Purohit, S. S, and S. K. Mathur. 1996. Biotechnology. Agro’s Botanical Pub., India. Razdan, M. K. 2003. An Introduction to Plant Tissue Culture. Oxford & IBH Pub. Co., Pvt. Ltd., New

Delhi. Spier, R. B. and J. B. Griffiths. 1990. Animal Cell Biotechnology, Academic Press, London. Turner, P. C., A. G. McLennan, A. D. Bates and M. R. H. White. 2002. Instant Notes: Molecular

Biology. 2nd Edition. Bios. Scientific. Pub. Ltd., Oxford, UK. Watson, J. D. 1992. Recombinant DNA, USA.

BIOTECHNOLOGY DEPARTMENT

Bangladesh Agricultural University, Mymensingh

Syllabus for B. Sc. Ag. (Hons.); Level-1; Semester-II Course No: BTech 122 Course Title: BASIC BIOTECHNOLOGY-Practical (Elective)

Credit hrs: 2, Contact hours: 2

  1. Biotechnology Laboratory design and requirements.

  2. Media and buffer preparation, aseptic manipulation, explant preparation.

  3. Node, meristem, embryo, pollen, anther, ovule, protoplast, cell and callus culture.

  4. Isolation of genomic DNA from plant, fish and animal tissues; isolation of plasmid DNA.

  5. DNA, RNA and protein electrophoreses.

  6. Digestion, ligation, competent cell preparation, transformation in Escherichia coli.

Text Books/References

Alberts, B., D. Bray, J. Lewis, M. Raff, K. Roberts and J. D. Watson. 1989. Molecular Biology of the Cell. 2nd Edition. Garland Pub., Inc., New York.

Brown, T. A. 2002. Gene Cloning and DNA Analysis. 4th Edition. Blackwell Pub., London.

Butler, M. 1991. Mammalian Cell Biotechnology-A Practical Approach, IRL Pub., Oxford.

Glover, D. M. and B. D. Hames. 1995. DNA Cloning-1 Core Techniques: A Practical Approach. Oxford University Press, UK.

Sambrook, J., E. F. Fritsch and T. Manniatis. 1999. Molecular Cloning. Vol. 1, 2, 3. Cold Spring Harbor Press, USA.

Gamborg. O. L. and G. S. Phillips. 1995. Plant Cell Tissue and Organ Culture. Fundamental Methods, Narosa Pub. House, New Delhi.

Jane, K. S. and H. Alexander. 1982. Genetics engineering. Principles and methods Plenum Press, New York.

Raymond, L. R. and C. T. Robert. 1983. Recombinant DNA Techniques. An Introduction. The Benjamin Cumming Pub., Co., London.

DEPARTMENT OF BIOTECHNOLOGY

Bangladesh Agricultural University, Mymensingh

Syllabus for B. Sc. Ag. (Hons.); Level-4; Semester-I & II Course No: BTech 401 Course Title: GENETIC

ENGINEERING AND BIOTECHNOLOGY-Theory (Elective)

Credit hrs: 2, Contact hours: 2

  1. Introduction: Concept, scope and importance of Genetic Engineering.

  2. Gene Manipulation: Recombinant DNA technology, vectors, methods for gene delivery, selection of recombinants, site specific recombination/mutagenesis.

  3. Molecular Techniques: Nucleic acid and protein purification, Electrophoresis, PCR, Southern, Northern and Western blotting; DNA sequencing, cDNA library.

  4. Gene Expression: Regulation of transcription in prokaryotes and eukaryotes, promoter, terminator, splicing and processing of RNAs, translation, Operon concept.

  5. Application of Genetic Engineering:

Plants: Pest resistance, herbicide tolerance, resistance to fungi, bacteria and virus. Oxidative, salt, drought and submergence tolerance. Nutritional quality improvement: Provitamin A, iron, protein etc. Genetic manipulation for flower pigmentation, nitrogen fixation, photosynthesis, male sterility, fruit ripening, senescence tolerance. Edible vaccines in food products.

Fish and Animals: Production of transgenic animal and fish; feed quality improvement; dairy products and milk quality, recombinant protein.

Medicine and Industry: Commercial synthesis of hormones, vaccines, gene therapy, disease diagnosis, monoclonal antibodies, biomining, biogas, bioengineering. genome mapping, DNA fingerprinting, forensic medicine; enzymology, immunotechnology.

  1. Biosafety and GMO: Biosafety and environmental issues. Biosafety guidelines & regulations. Ethics and issues regarding genetically modified organisms. Religious and social acceptance of GMOs.

  2. Bioinformatics: Model for Sequence related information, usage of IT in biotechnology, internet browsing for retrieval and dissemination of biological information.

Text Books/References

Baxevanis, A. D. and B. F. F. Ouellette. 2002. Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins. 2nd Edition. Gopsons Papers Ltd., India.

Cunningham, C. and A. J. R. Porter. 2000. Recombinant Proteins form Plants. Humana Press, New Jersey.

Gupta, P. K. 1997. Cell and Molecular Biology. Rastogi Pub., India.

Lewin, B. 2004. Genes VIII. Pearson Prentice Hall Pub., NJ 07458.

Mehga, P., D. F. Klessig, A. R. Oshmore, W. Cruissem, and J. E. Varner. 1995. Methods in Plants

Molecular Bioloty, Cold Spring Harbour Lab. Press, New York.

Michael, W. F., S. W. Graham and M. M. Young. 1992. Plant Biotechnology. Pergamon Press, New York.

Primrose, S.B. 1987. Modern Biotechnology. Blackwell Scientific Pub., London.

Primrose, S. B., R. M. Twyman and R. W. Old. 2003. Principles of Gene Manipulation. 6th Edition. Blackwell Pub. Com., Germany.

Traynor, P. L., R. J. Frederick and M. Koch. 2002. Biosafety and Risk Assessment in Agricultural Biotechnology.

Watson, J. D., T. A. Baker, S. P. Bell, A. Gann, M. Levine, R. Losick. 2004. Molecular Biology of the Gene. 5th Edition. Pearson Education Pte. Ltd., New Dilhi, India.

Westhead, D. R., J. H. Parish and R. M. Twyman. 2003. Instant Notes: Bioinformatics. 1st Edition. Bios. Scientific Pub. Ltd., Oxford, UK.

Winnacker, E. L. 2003. From Genes to Clones: Introduciton to Gene Technology. Panima Publishing Corporation, New Delhi.

BIOTECHNOLOGY DEPARTMENT

Bangladesh Agricultural University, Mymensingh

Syllabus for BSc. Ag. (Hons.); Level-4; Semester-I & II Course No: BTech 402 Course Title: GENETIC ENGINEERING AND BIOTECHNOLOGY-Practical (Elective)

Credit hrs: 2, Contact hours: 2

  1. Agrobacterium-mediated transformation techniques; co-cultivation of explants.

  2. Polymerase Chain Reaction (PCR), Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), Randomly Amplified Polymorphic DNA (RAPD); Southern, Northern and Western blotting; bioassay tests.

  3. Radioisotopes, their uses and monitoring concept, counting efficiency; autoradiography.

  4. Applications and types of chromatography.

  5. Applications of UV and visible spectrophotometry, fluorimetry.

  6. Cryopreservation and conservation of seed, plant sample, semen, embryo, revival of frozen germplasm.

Text Books/References

Ansubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. A. Sidman, J. A. Smith and K. Struhl. 1993. Current Protocols in Molecular Biology. Wiley Pub., UK.

Baxevanis, A. D. and B. F. F. Ouellette. 2002. Bioinformatics: A Practical Guide to the

Analysis of Genes and Proteins. 2nd Edition. Gopsons Papers Ltd., India.

Brown, T. A. 2002. Gene Cloning and DNA Analysis. 4th Edition. Blackwell Pub., London.

Glover, D. M. and B. D. Hames. 1995. DNA Cloning-1 Core Techniques: A Practical Approach. Oxford University Press, UK.

Jane, K. S. and H. Alexander. 1982. Genetics engineering. Principles and methods Plenum Press, New York.

Kjellssion, G., V. Simonsen, and K. Ammann. 1997. Methods for Risk Assessment of Transgenic Plants. Birkhauser Verlag, Germany.

Raymond, L. R. and C. T. Robert. 1983. Recombinant DNA Techniques. An introduction. The Benjamin Cumming Publishing Co., London.

Robert, J. 1987. Tissue Culture of Selected Tropical Fruit Plants; a handbook on the application of tissue culture of plant propagation. FAO, Rome.

Sambrook, J., E.F. Fritsch and T. Manniatis. 1999. Molecular Cloning. Cold Spring Harbor Press, USA.

Singh, B. P., U. Srivastava. 2004. Plant Genetic Resources in Indian Perspective: Theory and Practices. Directorate of Information and Publications of Agriculture. Indian Council of Agricultural Research, New Delhi.

Slater, R.J. 1990. Radioisotopes in Biology- A Practical Approach. Oxford University Press, UK.

 

  
 

Copyright © 2008 All rights reserve by bdbic.com